한국해양대학교

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점액세균 Sorangium cellulosum이 생산하는 신규 spiroketal 화합물, Spirodienal D의 구조 및 생물활성

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dc.contributor.author 이화선 -
dc.date.accessioned 2017-02-22T06:58:33Z -
dc.date.available 2017-02-22T06:58:33Z -
dc.date.issued 2011 -
dc.date.submitted 56959-08-26 -
dc.identifier.uri http://kmou.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002175786 ko_KR
dc.identifier.uri http://repository.kmou.ac.kr/handle/2014.oak/10092 -
dc.description.abstract Myxobacteria are a rich source of natural products with biological activity. In particular, strains belong to the genus Sorangium proved to be unusually rich in secondary metabolites. So far, about 100 different core structures and 500 variants have been characterized. The search for novel compounds strongly depends on the availability of novel strains. During the last 3 years, we have isolated 96 strains of Sorangium cellulosum from soil samples collected at different areas in Korea. The strains were all used in our screening for secondary metabolites. We looked for biological effects as well as for new signals in the HPLC-diode array analysis of the culture extracts. Previous studies in our lab have demonstrated that a newly isolated strain KM0141 produced three new antibiotics, each containing a spiroketal moiety. In our search for additional antibiotics from the strain, a co-metabolite named Spirodienal D(1) was observed in very low yield. Pooled wet cell mass and adsorbent Amberlite XAD-16 from several fermentations(about 350L) of S. cellulosum KM0141, were extracted with acetone. Spirodienal D(1) was isolated as colorless oil after extraction, solvent partitioning, and silica gel chromatography. The crude product was further purified by HPLC to give 3.9mg of 1. The structure of 1 was elucidated by 2D-NMR and mass spectroscopy. The relative stereochemistry of the spiroketal ring was determined on the basis of 1H-1H coupling constants and NOE correlations. Spirodienals A-D were evaluated for cytotoxic activity against several human cancer lines using doxorubicin as a reference by SRB method. All these compounds demonstrated potent cytotoxicities against human cancer cells, having IC50 values ranging from 0.053μM to 0.878 μM. Interestingly, the cytotoxic activities of Spirodienals A-D against human colon cancer cells(HCT-15) were 2 to 7 times stronger than that of doxorubicin in terms of IC50. -
dc.description.tableofcontents Ⅰ. 서 론 3 Ⅱ. 실험재료 및 방법 9 1. 실험기기 및 시약 9 1.1. 실험기기 9 1.2. 물리화학적 특성 측정 11 1.3. 용매 13 1.4. 시약 및 충진제 14 1.5. TLC(Thin layer chromatography) 16 2. 시험균 18 3. 배지조제 19 3.1. 항균활성 및 먹이균 배양을 위한 배지 19 3.2. Sorangium 균주의 분리 및 동정을 위한 배지 21 3.3. Sorangium 균주의 보관 및 액체배양을 위한 배지 27 4. 먹이균의 배양 29 5. Sorangium 균주의 분리 및 동정 30 5.1. 토양시료의 채집 및 처리 30 5.2. Sorangium 균주의 분리 및 순화 30 5.3. Sorangium 균주의 동정 34 6. Sorangium 균주의 배양 38 6.1. 고체배양 38 6.2. 액체배양 38 7. 대사산물의 추출 및 분획 40 7.1. 대사산물의 추출 40 7.2. 대사산물의 분획 40 8. 균주 KM0141이 생산하는 새로운 대사산물의 분리 42 9. 생물 활성 측정 45 9.1. 항균활성 45 9.2. 세포독성 46 Ⅲ. 실험결과 및 고찰 48 1. 분리된 Sorangium 균주의 동정 및 특성 48 1.1. Sorangium 균주의 분리 및 특성 48 1.2. 균주 KM0141의 특성 및 동정 53 2. Sorangium 균주들이 생산하는 대사산물의 스크리닝 59 3. KM0141이 생산하는 새로운 대사산물의 구조결정 64 4. 생물활성 75 4.1. 항균활성 75 4.2. 세포독성 76 Ⅳ. 결 론 78 참고문헌 81 부 록 86 -
dc.language kor -
dc.publisher 한국해양대학교 -
dc.title 점액세균 Sorangium cellulosum이 생산하는 신규 spiroketal 화합물, Spirodienal D의 구조 및 생물활성 -
dc.title.alternative Structure and Biological Activity of Spirodienal D, A New Spiroketal from the Myxobacterium Sorangium cellulosum. -
dc.type Thesis -
dc.date.awarded 2011-02 -
dc.contributor.alternativeName Hwa-Sun Lee -
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해양생명환경학과 > Thesis
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