한국해양대학교

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Dietary inclusion effect of various sources of phyto-additives on the growth performance of olive flounder (Paralichthys olivaceus), and challenge test against Edwardsiella piscicida

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dc.contributor.advisor 조성환 -
dc.contributor.author ZAMAN MD FARID UZ -
dc.date.accessioned 2024-01-03T17:28:35Z -
dc.date.available 2024-01-03T17:28:35Z -
dc.date.created 2023-03-03 -
dc.date.issued 2023 -
dc.identifier.uri http://repository.kmou.ac.kr/handle/2014.oak/13116 -
dc.identifier.uri http://kmou.dcollection.net/common/orgView/200000670023 -
dc.description.abstract Inclusion of various sources of additive in aquafeeds is practically helpful to elevate growth performance and disease resistance of aquatic animals. An 8-week feeding trial was conducted to determine inclusion effects of phyto-additives (yacon, ginger, and blueberry) into diets on olive flounder growth, feed utilization, chemical composition, and plasma chemistry, and challenge test against Edwardsiella piscicida in comparison to a commercial probiotic (Super lacto®). Six hundred juvenile olive flounder were randomly assigned into fifteen, 50 L flow-through tanks (40 fish per tank). Five experimental diets were formulated. No additive was included in the control (Con) diet. One percent of yacon, ginger, and blueberry powders, and 0.5% Super lacto® were included at the expense of wheat flour, and water in the Con diet referred to as the YCP, GGP, BBP, and SUP diets, respectively. All experimental diets were hand-fed to triplicate groups of fish twice a day until they visually satiated for a period of 8 weeks. Following the 4- and 8-week feeding trials, 10 and 20 fish were taken from each tank, artificially infected with E. piscicida, and then survival was monitored for the next 6, and 8 days after infection, respectively. At the end of the 8-week feeding trial, no significant difference was observed in weight gain, feed consumption, feed utilization, chemical composition, and plasma chemistry of olive flounder. However, survival of fish fed the GGP diet was significantly (p < 0.004, and p < 0.02, respectively) higher than that of fish fed the SUP, BBP, and Con diets, and SUP and Con diets after the 4- and 8-week feeding trials, respectively, according to the Kaplan-Meier survival curve. In conclusion, phyto-additive, especially GG can be used as an effective immunostimulant in olive flounder diet at occurrence of E. piscicida.|배합사료 내 사료첨가제를 공급하는 것은 수생동물의 성장과 질병 저항성을 향상시키는데 실질적인 도움이 된다. 다양한 식물성 원료인 야콘, 생강과 블루베리의 첨가에 따른 넙치의 성장, 사료이용성, 체조성, 혈액학적 성상 및 Edwardsiella picicida 공격성에 미치는 효과를 상업용 프로바이오틱스(슈퍼락토)와 비교하기 위하여 8주간의 사육실험을 수행하였다. 총 600 마리의 넙치 치어를 15개의 50 L 유수식수조에 각각 40마리씩 무작위로 분산 수용하였다. 대조구사료(Con)를 포함한 5종류의 실험용 배합사료를 준비하였다. 대조구사료는 사료 내 어떠한 사료첨가제도 포함시키지 않았으며, 대조구사료 내 소맥분과 물을 각각 1%의 야콘, 생강과 블루베리 분말 및 0.5% 슈퍼락토로 대체하여 제조하고, 이를 각각 YCP, GGP, BBP 및 SUP 사료로 명명하였다. 각 실험사료는 3반복구를 두었다. 모든 실험사료는 손으로 매일 1일 2회 만복시까지 공급하였으며, 총 8주간 공급하였다. 사육실험 시작 후, 4주차와 사육 실험이 종료된 8주차에는 각 수조에서 10마리와 20마리의 넙치 치어를 무작위로 선택하여 E. piscicida로 인위적인 감염을 시켰으며, 감염 이후 6일과 8일 동안 각각의 생존율을 관찰하였다. 8주간의 사육실험 종료 시, 어체 증체량, 일일성장율, 사료 공급양, 사료이용성, 체조성 및 혈액학적성상에는 실험구간에 유의적인 차이가 없었다. 그러나, Kaplan-Meier 생존율 곡선 분석에 따르면, 4주 사육실험 종료 시에는 GGP사료를 공급한 넙치의 생존율은 SUP사료, BBP사료와 대조구사료를 공급한 넙치보다 높은 생존율을 보였다. 또한 8주간의 사육실험 시에도 GGP사료를 공급한 넙치의 생존율이 SUP사료와 대조구사료를 공급한 넙치보다 높았다. 이상의 결과를 고러할 때, 식물성 사료첨가제, 특히 생강의 첨가는 넙치용 배합사료내 E. piscicida 발병으로 인한 넙치의 폐사율을 감소시키는데 아주 효과적인 것으로 판단된다. -
dc.description.tableofcontents 1. Introduction 1 2. Materials and Methods 5 2.1 Collection of fish and rearing conditions 5 2.2 Experimental diet preparation 5 2.3 Measuring performance of the experimental fish 8 2.4 Chemical analysis of the experimental diets and fish 8 2.5 Analysis of plasma chemistry of the experimental fish 8 2.6 Challenge test 9 2.7 Statistical analysis 9 3. Results 10 3.1 Survival and growth performance of the fish at the end of the 8-week feeding trial 10 3.2 Feed consumption and feed utilization of the fish 10 3.3 Chemical composition of the whole body of fish 10 3.4 Plasma chemistry of fish at the end of the 8-week feeding trial 10 3.5 Challenge test of the fish at the end of the 4- and 8-week feeding trials 15 4. Discussion 18 5. Conclusion 23 Acknowledgements 24 References 25 Abstract (in Korean) 36 -
dc.format.extent 45 -
dc.language eng -
dc.publisher 한국해양대학교 해양과학기술전문대학원 -
dc.rights 한국해양대학교 논문은 저작권에 의해 보호받습니다. -
dc.title Dietary inclusion effect of various sources of phyto-additives on the growth performance of olive flounder (Paralichthys olivaceus), and challenge test against Edwardsiella piscicida -
dc.type Dissertation -
dc.date.awarded 2023-02 -
dc.embargo.terms 2023-03-03 -
dc.contributor.department 해양과학기술전문대학원 해양과학기술융합학과 -
dc.contributor.affiliation 한국해양대학교 해양과학기술전문대학원 해양과학기술융합학과 -
dc.description.degree Master -
dc.identifier.bibliographicCitation ZAMAN MD FARID UZ. (2023). Dietary inclusion effect of various sources of phyto-additives on the growth performance of olive flounder (Paralichthys olivaceus), and challenge test against Edwardsiella piscicida. -
dc.subject.keyword Olive flounder (Paralichthys olivaceus), Phyto-additive, Edwardsiella piscicida, Immunostimulant, Kaplan-Meier survival curve -
dc.contributor.specialty 사료영양학 -
dc.identifier.holdings 000000001979▲200000003272▲200000670023▲ -
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