한국해양대학교

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Archangium gephyra가 생산하는 Diketopiperazine 화합물

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dc.contributor.author 김민선 -
dc.date.accessioned 2017-02-22T02:16:47Z -
dc.date.available 2017-02-22T02:16:47Z -
dc.date.issued 2008 -
dc.date.submitted 2008-01-25 -
dc.identifier.uri http://kmou.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002174177 ko_KR
dc.identifier.uri http://repository.kmou.ac.kr/handle/2014.oak/8131 -
dc.description.abstract Myxobacteria are very remarkable microorganisms which are usually not obtained by standard isolation technique. These organisms form characteristic fruiting bodies which are not produced by other bacteria. Although not much is known about the basic physiology and biochemistry of these bacteria. They have proved to produce a variety of bioactive substances. A great portion of these substances inhibit antifungal or cytotoxic properties. In the course of screening for new bioactive substances from myxobactera, strain KM593 was found to produce closely related diketopiperazines(cyclic dipeptides). The strain was identified as Archangium gephyra by morphological characteristics and staining with Congo red. The isolation of diketopiperazines was performed through several steps including silica gel, RP 18, Sephadex LH-20 column chromatography and HPLC. The chemical structures of these compounds were established on basis of their 1-D and 2-D NMR spectroscopic analyses and by comparison with reference data from literature. They were assigned as Cyclo(L-Pro-L-Tyr), Cyclo(L-Pro-L-Phe), Cyclo(D-Pro-L-Tyr), Cyclo(L-Pro-L-Leu), Cyclo(L-Pro-D-Val), respectively. Antioxidant activities of these compounds were investigated. In particular, Cyclo(L-Pro-L-Tyr) showed higher antioxidant activity than Trolox in terms of ORAC values. Diketopiperazines are most commonly isolated from terrestrial yeasts, lichens and fungal culture filtrates and are also observed in the culture broth of marine bacteria and marine actinomycetes. This is the first report that diketopiperazines were produced by myxobacteria. -
dc.description.tableofcontents LIST OF TABLES LIST OF FIGURES LIST OF ABBREVIATIONS AND SYMBOLS ABSTRACT Ⅰ. 서론 Ⅱ. 실험 재료 및 방법 1. 실험 재료 1.1 시약 1.2 기기 1.3 용매 1.4 시험균 1.5 배지조성 2. 실험 방법 2.1 용균성 점액세균의 분리 및 배양 2.2 대사산물의 추출 및 분리 정제 2.3 생물활성측정 Ⅲ. 결과 및 고찰 1. 점액세균의 분리 1.1 점액세균의 배양과 대사산물의 스크리닝 2. 균주 KM593의 분류와 동정 3. Archangium gephyra KM593이 생산하는 활성물질의 분리 3.1 활성물질의 구조결정 3.2 분리된 화합물(1~5)의 생리활성 Ⅳ. 결론 Ⅴ. 참고문헌 부록 -
dc.language kor -
dc.publisher 한국해양대학교 대학원 -
dc.title Archangium gephyra가 생산하는 Diketopiperazine 화합물 -
dc.title.alternative Diketopiperazine compounds produced by Archangium gephyra -
dc.type Thesis -
dc.date.awarded 2008-02 -
dc.contributor.alternativeName Min-Sun Kim -
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해양생명환경학과 > Thesis
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