한국해양대학교

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Differential Daily Rhythms of Melatonin in the Pineal Gland and Gut of Goldfish, Carassius auratus in Response to Light

DC Field Value Language
dc.contributor.author 최지용 -
dc.date.accessioned 2017-02-22T02:19:34Z -
dc.date.available 2017-02-22T02:19:34Z -
dc.date.issued 2016 -
dc.date.submitted 2016-03-12 -
dc.identifier.uri http://kmou.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002231639 ko_KR
dc.identifier.uri http://repository.kmou.ac.kr/handle/2014.oak/8214 -
dc.description.abstract The objectives of this study were to test the effects of light on melatonin rhythms in the pineal gland and gut of goldfish (Carassius auratus) and to investigate whether melatonin function differed in these two tissues, which are respectively photosensitive and non-photosensitive. Rhythms were evaluated by measuring aralkylamine N-acetyltransferase (AANAT2) and melatonin receptor 1 (MT-R1) mRNA expression and melatonin concentration in the pineal gland, gut (in vivo), and cell cultures of the two tissues (in vitro). Compared to control, pineal gland melatonin secretion was higher at night, whereas the 24-h dark and ophthalmectomy groups maintained higher AANAT2 and MT-R1 mRNA expression during the day. Melatonin levels and AANAT2 and MT-R1 mRNA expression in the gut were also the highest at night, but the 24-h light, dark, and ophthalmectomy groups did not significantly differ from control. Furthermore, we measured AANAT2 and MT-R1 mRNA expression in high temperature water (30 °C) to investigate differences in the antioxidant capacity of pineal gland versus gut melatonin. Melatonin and H2O2levels,aswellasAANAT2 and MT-R1 mRNA expression, were all higher in the two tissues under thermal stress, compared with their levels at 22 °C. Taken together, our results suggest that light has no effect on melatonin patterns in the gut, which appears to exhibit its own circadian rhythm, but both gut and pineal gland melatonin exhibit similar antioxidant function. -
dc.description.tableofcontents Contents List of Tables List of Figures List of Abbreviations Abstract (in Korean) Abstract 1. Introduction 2. Materials and methods 2.1. Experimental fish, ophthalmectomy, and conditions 2.2. In vitro culture of pineal gland and whole gut cells 2.3. Quantitative PCR (QPCR) 2.4. Western blot analysis 2.5. Melatonin concentration level 2.6. H2O2 concentration measurement 2.7. Statistical analysis 3. Results 3.1. Change of pineal gland AANAT2 expression levels 3.2. Change of whole gut AANAT2 expression levels 3.3. Change of pineal gland MT-R1 protein and mRNA expression levels 3.4. MT-R1 protein and mRNA expression levels 3.5. Melatonin concentration 3.6. AANAT2 and MT-R1 expression in response to thermal stress 3.7. H2O2 concentration under thermal stress conditions 4. Discussion Acknowledgements References -
dc.language eng -
dc.publisher 한국해양대학교 대학원 -
dc.title Differential Daily Rhythms of Melatonin in the Pineal Gland and Gut of Goldfish, Carassius auratus in Response to Light -
dc.title.alternative 빛 조절에 의한 금붕어, Carassius auratus 송과체와 장 멜라토닌의 일주기 리듬 차이 -
dc.type Thesis -
dc.date.awarded 2016-02 -
dc.contributor.alternativeName Ji Yong Choi -
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해양생명환경학과 > Thesis
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