건조 고등어의 화학성분 및 생리활성

Abstract

This study investigated chemical components and biological activities (anticancer and antioxidant effects) of mackerel dried by low temperature vacuum method. In order to determine the inhibitory effects of solvent extracts from dried mackerel on growth of cancer cell lines (AGS human gastric adenocarcinoma, HT-29 human colon cancer and HT-1080 human fibrosarcoma cells), MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was conducted. Inhibitory effects of acetone with methylene chloride (A+M) and methanol (MeOH) extracts on the growth of AGS, HT-29 and HT-1080 cancer cells increased in a dose dependent manner (p<0.05). The treatments of n-hexane, 85% aq. MeOH, n-butanol and water fractions significantly inhibited the growth of cancer cells (p<0.05). Among the fractions, 85% aq. MeOH fraction showed a higher inhibitory effect on growth of cancer cell lines. DCHF-DA (dichlorodihydrofluorescin diacetate) assay was conducted to determine the protective effect of dried mackerel on H2O2-induced oxidative stress. Levels of intracellular reactive oxygen species (ROS) were measured in a cellular system using HT-1080 cell line. All fractions including crude extracts of dried mackerel significantly reduced the production of ROS in a dose-dependent manner (p<0.05). Among the fractions, 85% aq. MeOH fractions showed the highest protective effect on production of lipid peroxides. In addition, n-Hexane and 85% aq. MeOH fractions from dried mackerel increased levels of intracellular glutathione (GSH) in a dose dependent manner. In case of nitric oxide (NO) reduction assay, A+M extract, n-hexane and 85% aq. MeOH fractions reduced NO production by 38%. In conclusion, these results suggest that 85% aq. MeOH fractions may be developed as a candidate for potential antioxidant related to oxidative stress, and that the intake of mackerel may be recommended as a valuable food for preventing oxidative stress and reducing cancer risk.