한국해양대학교

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세루로즈 용해성 점액세균 Sorangium cellulosum의 분리 및 대사산물에 관한 연구

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dc.contributor.author 유태경 -
dc.date.accessioned 2017-02-22T06:23:56Z -
dc.date.available 2017-02-22T06:23:56Z -
dc.date.issued 2008 -
dc.date.submitted 56877-07-05 -
dc.identifier.uri http://kmou.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002175283 ko_KR
dc.identifier.uri http://repository.kmou.ac.kr/handle/2014.oak/9468 -
dc.description.abstract Myxobacteria are unique gram-negative bacteria characterized by the gliding and fruiting body forming nature. They have recently been well established as a new and potent source for natural products with biological activities because of their potential to produce a considerable variety of metabolites.1-4) Thus, myxobacteria especially attract many researchers, since they have many possibilities of producing undiscovered bioactive substances.5) They are not obtained by the routine method used in culturing bacteria and thus require special techniques for their isolation and culture. We established in these techniques about Sorangium cellulosum in Korea. In our search for the bioactive metabolites from myxobacteria, strains KM1001 and KM1041 of Sorangium cellulosum, were found to produce potent bioactive compounds. They were isolated and purified by silica gel column chromatography and semi-preparative HPLC. The structures of these compounds were determined on the basis of combined spectroscopic analysis. Epothilone A (1)6-8) from S. cellulosum KM1041 shown to have a significant cytotoxicity against the HM7 and HeLa human cancer cell lines with IC50 values 20 and 30nM. At higher concentrations of epothilon A, the microtubles became arranged in bundle-like formations at the periphery of the cell.9-13) The major component from S. cellulosum KM1001, named soraphinol C(4)14-16) was found to be a new compound closely related to the minor component, 4-hydroxysattabacin (5).17) Soraphinol C (4) showed antioxidant activity comparable to that of Trolox, while 4-hydroxylsattabacin (5) possessed only a weak antioxidant activity. -
dc.description.tableofcontents Abstract Ⅰ. 서론 Ⅱ. 실험재료 및 방법 1. 세루로즈 용해성 점액세균의 분리 및 배양 1.1 세루로즈 용해성 점액세균의 분리와 순화 1.2 세루로즈 용해성 점액세균의 배양 2. 대사산물의 추출 및 분리 정제 2.1 추출 및 분획 2.2 분리 정제 2.3 기기분석 3. 생물활성측정 3.1 항균활성 3.2 항산화활성 (ORAC assay) 3.3 세포독성 Ⅲ. 결과 및 고찰 1. 세루로즈 용해성 점액세균의 분리 및 동정 1.1 세루로즈 용해성 점액세균의 분리 1.2 세루로즈 용해성 점액세균의 동정 2. 세루로즈 용해성 점액세균의 대사산물의 스크리닝 3. S. cellulosum KM1041의 대사산물의 분리정제, 구조결정 및 생물활성 4. S. cellulosum KM1001의 대사산물의 분리정제, 구조결정 및 생물활성 Ⅳ. 결 론 Ⅴ. 부 록 Ⅶ. 참고 문헌 -
dc.language kor -
dc.publisher 한국해양대학교 대학원 -
dc.title 세루로즈 용해성 점액세균 Sorangium cellulosum의 분리 및 대사산물에 관한 연구 -
dc.title.alternative Isolation and structure elucidation of bioactive secondary metabolites of Sorangium cellulosum, cellulose-degrading myxobacteria -
dc.type Thesis -
dc.date.awarded 2008-02 -
dc.contributor.alternativeName Yu Tae Kyoung -
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해양생명환경학과 > Thesis
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