한국해양대학교

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토양 조건에서의 천연유기물 첨가에 의한 Endosulfan 생분해 촉진효과 규명 및 PCNB 분해균의 분리ㆍ동정

Title
토양 조건에서의 천연유기물 첨가에 의한 Endosulfan 생분해 촉진효과 규명 및 PCNB 분해균의 분리ㆍ동정
Alternative Title
Effect of Natural Organic Substrates on Facilitation of Endosulfan Bildegradation in a Soil and Isolation of PCNB degraders
Author(s)
愼晟揆著
Publication Year
2003
Publisher
한국해양대학교 대학원
URI
http://kmou.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002174043
http://repository.kmou.ac.kr/handle/2014.oak/10410
Abstract
Endosulfan and pentachloronitrobenzene (PCNB) are toxic pesticides that were used to control soil-borne plant diseases but are still detected in soils and ediblecrops despite its terminated use. In this study we report a new Endosulfan and PCNB-degrading strains able to grow under ambient aerobic conditions. These strains could readily utilize Endosulfan and PCNB (100ppm) as a sole carbon source respectively and showed a distinctive growth in a week or so. Endosulfan degrader was identified as Klebsiella oxytoca KE-8 and PCNB degrader as Alcaligenes denitrificans based upon 16S rDNA sequence analysis. In this study a PCR-DGGE (denaturing gradient gel electrophoresis) system was developed to specifically monitor endosulfan or PCNB degrader population (Klebsiella oxytoca KE-8 and Alcaligenes denitrificans). The isolation and characterization of the new degraders will be useful in facilitating accelerated bioremediation of Endosulfan and PCNB in the environment and in monitoring the bioremediation processes.
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토목환경공학과 > Thesis
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