Endosulfan and pentachloronitrobenzene (PCNB) are toxic pesticides that were used to control soil-borne plant diseases but are still detected in soils and ediblecrops despite its terminated use. In this study we report a new Endosulfan and PCNB-degrading strains able to grow under ambient aerobic conditions. These strains could readily utilize Endosulfan and PCNB (100ppm) as a sole carbon source respectively and showed a distinctive growth in a week or so. Endosulfan degrader was identified as Klebsiella oxytoca KE-8 and PCNB degrader as Alcaligenes denitrificans based upon 16S rDNA sequence analysis. In this study a PCR-DGGE (denaturing gradient gel electrophoresis) system was developed to specifically monitor endosulfan or PCNB degrader population (Klebsiella oxytoca KE-8 and Alcaligenes denitrificans). The isolation and characterization of the new degraders will be useful in facilitating accelerated bioremediation of Endosulfan and PCNB in the environment and in monitoring the bioremediation processes.