한국해양대학교

Detailed Information

Metadata Downloads

미꾸라지, Misgurnus mizolepis 간조직의 expressed sequence tags (ESTs) 분석 및 estradiol-17┑ 처리에 의한 발현 특징

DC Field Value Language
dc.contributor.author 이수진 -
dc.date.accessioned 2017-02-22T06:05:12Z -
dc.date.available 2017-02-22T06:05:12Z -
dc.date.issued 2005 -
dc.date.submitted 56823-03-29 -
dc.identifier.uri http://kmou.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002174935 ko_KR
dc.identifier.uri http://repository.kmou.ac.kr/handle/2014.oak/9066 -
dc.description.abstract Expressed sequence tags (ESTs) represent the transcribed fraction of complementary DNA from organism's cDNA library. Randomly selected 910 clones were sequenced from mud loach, Misgurnus mizolepis G㉨nther, 1888 cDNA library constructed using mRNA extracted from liver. Of 910 mud loach liver cDNA clones analyzed into similarity that 94.9% clones significant similarity with sequeces from GenBank (e-value<10-3) and 6.2% showed poor similarity (e-value◎10-3) or no matched any sequence in GenBank. The percentage of singleton (unique sequence) represented 38.6% in this ESTs database. Analysis of organisms were stand for the best hit in this ESTs showed that 82.6% matched with fish entries. Functional clustering of this ESTs based on gene expression. Functional groups consisted of 10 classes. In this libraries, the class ?? (immune function, chaperones and defense mechanism) and class ?? (general metabolism) was the largest groups with an average of 18.5% and 18.9%, respectively. We selected 467 unigene clones from mud loach ESTs which was fundamental basis of this database. Mud loach male were sex reversed using estradiol-17┑(E2). The survival of fish during treatment was dose and duration independent. But, growth performance were unaffected dose and duration. Acute symptoms showed that exposure to E2 for short times and affected death. On the other hand, chronical symptoms revealed gall-bladder different in treatment groups that exposure to E2 for long times. Morphologically, however, they were not changed liver and gonad. cDNA microarray signal revealed relation to stress and physiological unbalance genes that highly increase expression than other genes. Conversely, relation to general metabolism genes were highly decrease expression than other genes. Consequently, the objective of this study was to analysis of gene expression in mud loach liver, cDNA microarray using this EST database approach to survey of the effects of pollutant E2 on an evrionmentally relevant, male mud loach observation of changed histology and morphology. -
dc.description.tableofcontents I. 서론 1 Chapter I. 미꾸라지 간조직의 Expressed Sequence Tags 탐색 및 데이터... 4 II. 재료 및 방법 4 1. 실험어 4 2. Total RNA 및 mRNA 분리 4 3. 미꾸라지 간조직의 cDNA library 제작 5 4. 미꾸라지 간조직의 ESTs 분석을 위한 cDNA 확보 6 5. 염기서열 분석 및 ESTs 데이터베이스 구축 7 II. 결과 8 1. cDNA library 제작 및 평가 8 2. 상동성에 의한 미꾸라지 ESTs 정보에 대한 평가 8 3. 미꾸라지 ESTs의 redundancy 평가 11 4. 생물종에 의한 미꾸라지 ESTs 평가 14 5. 미꾸라지 ESTs의 기능별 clustering 및 데이터베이스 구축 14 Chapter II. Estradiol-17┑에 노출된 수컷 미꾸라지 간조직 유전자들의... 18 II. 재료 및 방법 18 1. 실험어 18 2. Estradiol-17┑ 처리 18 3. 생존률, 성장률 및 비만도 분석 19 4. 외형과 내부 장기 관찰 및 간과 생식소의 조직학적 조사 20 5. 간조직의 ESTs 클론을 이용한 cDNA microarray 제작 20 6. Total RNA 분리, probe 제작, hybridization 및 signal 분석 21 III. 결과 22 1. Estradiol-17┑에 노출된 수컷 미꾸라지의 생존률, 성장률 및 비만도 22 2. Estradiol-17┑에 노출된 수컷 미꾸라지의 외형과 내부 장기 및 간과... 22 3. 미꾸라지 간조직의 cDNA microarray를 이용한 발현 특징 31 IV. 고찰 34 V. 국 문 요 약 39 참 고 문 헌 43 Expressed sequenc -
dc.language kor -
dc.publisher 한국해양대학교 대학원 -
dc.title 미꾸라지, Misgurnus mizolepis 간조직의 expressed sequence tags (ESTs) 분석 및 estradiol-17┑ 처리에 의한 발현 특징 -
dc.title.alternative Exprssed sequence tags (ESTs) analysis of mud loach, Misgurnus mizolepis liver and expression during experimental exposure to estradiol-17┑ -
dc.type Thesis -
dc.date.awarded 2005-02 -
dc.contributor.alternativeName Soo Jin Lee -
Appears in Collections:
해양생명환경학과 > Thesis
Files in This Item:
000002174935.pdf Download

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse