서해안 조간대에 서식하는 해양 생물 및 저질 내에 농축되어 있는 다환 방향족 탄화수소 분석

Abstract

The High Performance Liquid Chromatography (HPLC)

Analysis of Polycyclic Aromatic Hydrocarbons (PAHs)

in Marine Organisms and sediments

from the Intertidal Zone of Yellow Sea, Korea

Porycyclic Aromatic Hydrocarbons (PAHs) are ubiquitous environmental pollutant. Because of the high toxicity of some polycyclic compounds, such as benzo(a)pyrenes, the determine of their level in sediment and marine organisms was target of several papers.

The basic purpose of this study is reporting preliminary data on sediment, and marine organisms from the intertidal zone of West Sea. And our results of study compared with present studies results from intertidal zone of East sea and South Sea. PAHs are determined using by High Performance Liquid Chromatography (HPLC)in sediment, green larver (Ascidian), and Oyster (Ruditapes Philippinarum) of intertidal zone of West Sea. Sixteen PAHs compounds were identified, including carcinogenic compouds.

PAHs in samples (sediment, green larver and oyster) were extracted and analyzed. 20g of each organisms (wet weight) and sediment (dry weight) was taken to be analyzed. These samples were Soxhlet-extracted and, then saponified by refluxing and then the PAHs were identified and quantified by HPLC.

Total PAHs in sediments from intertidal zone of West Sea ranged from 0.0077 to 1.6593 ppb (ng/g) (mean value, 0.4856±0.5273 ppb), in green larver ranged from 0.1490 to 1.1376 ppb (mean value, 0.4978±0.5539 ppb), in oyster ranged from 0.1675 to 2.8248 ppb (mean value, 0.9618±0.7441 ppb).

Our study area more polluted from intertidal zone of East Sea (Uljin, Youngdok, Kori), but, unpolluted from intertidal zone of South Sea (Kyoungyang, Pusan, Massan, Hadong).

The mean value of ratio of Σ≤ 3-rings to Σ ≥ 4-rings of sediments, green larver, and oyster was 0.5670±0.2230 (ranged between 0.000 and 3.657), 3.55±1.5657 (ranged between 0.092 and 6.138) and 0.4872±0.2829 (ranged between 0.3872 and 1.9496).

Compared with present other studies, we concluded that the PAHs level in intertidal zone of West Sea appeared to be comparatively low.